Recruitment of TRADD, FADD, and Caspase 8 to Double-Stranded RNA-Triggered Death Inducing Dignaling Vomplexes (dsRNA-DISCs)
Rapid elimination of virus-infected cells by apoptosis is an efficient anti-viral strategy. Double-stranded RNA (dsRNA), a viral product, is potently and rapidly apoptogenic in susceptible cells. Caspase 8 plays an important role in the dsRNA-induced apoptosis; however, the mechanisms of caspase 8 activation in response to dsRNA are unknown. We demonstrate here that, in HeLa cells, the dsRNA-triggered activation of caspase 8 is independent of ongoing proteins synthesis (and is, therefore, independent of changes in pro- and anti-apoptotic gene expression) and involves the formation of multiprotein dsRNA-triggered death inducing signaling complexes (dsRNA-DISCs). DsRNA-DISCs contain FADD, TRADD, and caspase 8; however, several experimental approaches suggest that death ligands and death receptors (such as Fas/Apo1 and DR4/Apo2) are not involved in the formation of dsRNA-DISCs.
Iordanov, M. S.; Kirsch, J. D.; Ryabinina, O. P.; Wong, J.; Spitz, P. N.; Korcheva, V. B.; Thorburn, A.; and Magun, B. E., "Recruitment of TRADD, FADD, and Caspase 8 to Double-Stranded RNA-Triggered Death Inducing Dignaling Vomplexes (dsRNA-DISCs)" (2005). Faculty Research. 19.