Evaluation of a Biomimetic Artificial Photolyase: Repair of Duplex DNA Containing a CPD Photolesion
The formation of cyclobutane pyrimidine dimers (CPD) by UV radiation is thought to be the first event in a cascade leading to skin cancer. Humans rely on nucleotide excision repair to repair this damage while several other species possess a photoreactivating enzyme (DNA photolyase) which is able to repair the CPD by binding to the damaged strand and effecting repair through electron transfer catalysis. Therefore, it is desirable to create a small molecule that is capable of repairing this photolesion. In this study we present the first catalytic repair of dsDNA containing a CPD through artificial means. This “artificial photolyase” is capable of binding CPDs and effect repair in much the same way as is seen with DNA photolyase. The effect of base flipping on the reaction equilibrium of this catalytic reaction is also discussed.
Johnson, Andrew T.; Underdue, Morgan; and Wiest, Olaf, "Evaluation of a Biomimetic Artificial Photolyase: Repair of Duplex DNA Containing a CPD Photolesion" (2010). Faculty Research. 42.